A CD4/Foxp3/OX40 triple immunofluorescence assay determines association between T cell immune subsets and outcome in colorectal cancer

نویسندگان

  • James Ziai
  • Mahrukh Huseni
  • Oded Foreman
  • Jeffrey Eastham-Anderson
  • Yuanyuan Xiao
  • Felix Chu
  • Marcin Kowanetz
  • Priti S Hegde
  • Jeong Kim
چکیده

Purpose Increased numbers of OX40+ T cells in the tumor microenvironment of colorectal cancer (CRC) patients have been associated with improved outcome. However, the OX40+ T cell population is heterogeneous and includes, among others, CD4+Foxp3+ regulatory T cells (Treg) as well as CD4+Foxp3effector T cells (Teff). In this study, we used a novel triple immunofluorescence assay for CD4, FoxP3 and OX40 to determine the relationship between tumor-associated CD4+ T cell subsets and patient disease stage as well as outcome in CRC.

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عنوان ژورنال:

دوره 2  شماره 

صفحات  -

تاریخ انتشار 2014